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KMID : 0380420100340030245
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Journal of Prventive Veterinary Medicine
2010 Volume.34 No. 3 p.245 ~ p.254
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Multi-residue method for simultaneous determination of sulfonamide residues in milk by Liquid chromatography with tandem mass spectrometry
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Lim Chae-Mi
Kwon Hyun-Jung
Cho Byung-Hoon
Park Su-Jeong
Chung Gab-Soo
Son Seong-Wan
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Abstract
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A simple, selective and sensitive procedure for the confirmation of 14 sulfonamide antibacterials in milk was developed. The milk samples were homogenized, extracted and deproteinized by acetonitrile and defatted by n-hexane. Analysis was performed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) in positive mode for all 14 analytes. Mass spectral acquisition was performed in the multi reaction monitoring mode (MRM), selecting two structurally significant transitions per compound. The calibrations were performed in sample matrixes and the interference effect of sample matrixes on the ionization was effectively eliminated. Good linear relationship (R2=0.992～ 0.999) were observed at 6 concentrations of 2.5～100 ng/g. Satisfied recoveries (86.3～110.2%) of all sulfonamides were demonstrated in spiked milk at three levels from 5 to 20 ng/g. The limits of quantitation (LOQs) for sulfonamides ranged from 0.25～2.1 ng/g.
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KEYWORD
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Sulfonamides, LC-MS/MS, Multi-Residue, Milk
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